The victim of a cruel, barbaric, and bloody murder in 1916, Edwin Payne's spirit was processed through The Tunnel of Souls, allowing the teenager to pass on to the Afterlife. Entering Death's domain, he was sentenced - thanks to a technicality - to an eternity in Hell, under the watchful eye of The Night Nurse. Terrified of the matron and her bed checks, he spent decades trying to escape. He also grew to fear the personification of mortality herself, despite her reportedly pleasant disposition.
During these escape attempts, he kept fleeing an invisible, shrieking entity down a never-ending corridor, fearing that they would one day catch up to him. Even after finally absconding back to the mortal plane, the memory and trauma he experienced still haunted him, causing him intense physical and emotional distress.
Making the acquaintance of Charles Rowland, the two became best friends, bonding over their mutual interest in detective novels. Feeling that they had too much spare time on their hands and wishing to help people, the boys created The Dead Boy Detective Agency, where Payne used his extensive knowledge of the Afterlife and paranormal entities to aid them in cases.
Despite all the good they did over the next forty years; he still feared the day that Death would one day track them down and bring them back to the Afterlife. Because of this, he insisted on setting out clear rules for their investigations; no crossing over to the realm of the dead and no performing any action that would attract the attention of their pursuer’s followers.
As time went on, Payne began developing strong romantic feelings for his friend and partner, something that he continually repressed due to his strict upbringing and the belief that this affection was "not normal" and wrong. Struggling to even address the subject, his love and adoration manifested itself in playful teasing and sarcasm, as well as protective concern for Rowland's wellbeing, particularly in cases related to his almost debilitating phobia of water, the cause of his death.
After helping to exorcise a sadistic demon from the medium Crystal Palace, he welcomed the psychic into their ranks, forming a loving, if not dysfunctional found family unit to call his own. They would regularly argue with one another over their approach to work.
Fond of the newest addition to the team, he did his best to hide his feelings, similarly as he did with Charles, by joking that they should kill her to ensure that she was safe as the only member of the Detective Agency that could die. However, these jokes often backfired, causing her to become angry and hurt at the suggestion, much to his reproach.
In 2021, while squabbling with Palace over their latest case, they are interrupted by the arrival of Danny the Ambulance. Although hesitant to drop what they are doing mid-investigation, he agrees to go with them, moved by Rowland's insistence that Danny was someone that they could not only trust but was also great to be around.
Travelling to Niles Caulder's Mansion on the outskirts of Covington, Ohio, Edwin introduced the group to Dorothy Spinner and Negative Man (Larry Trainer), their latest clients. Despite their rocky start, having been overheard insulting the pair with rude and derogatory comments about their looks, Spinner and Trainer agreed to team up with them to help resurrect their fallen friends; Cyborg (Victor Stone), Robot Man (Cliff Steel), Crazy Jane (Kay Challis), and Elasti-Girl/Woman (Rita Farr).
Discovering that they were still being processed in the Tunnel of Souls, Edwin initially dismissed the case, reminding Crystal of their number one rule. However, after Charles and Palace protested the decision, he reluctantly relented.
Crossing over to the other side with Larry in tow, astral projecting his spirit with the aid of Crystal, he acted as a guide to the afterlife. Growing impatient with the latter's innocuous and light commentary, Payne expressed the dire nature of their situation and the need for caution. Pushed further about Death, he went on to add that she was a fearsome force and not someone to be trifled with.
Finally reaching their destination, Edwin opened a portal to the afterlife, using his memories of the corridors and the pain they induced as payment. However, he was touched by Larry's offer to use his own loss in his stead.
As Rowland geared himself up to cross the River Styx on Charon's Ferry, Trainer, seeing many similarities to his younger, closeted self, decided to have a heart-to-heart with Payne about his feelings for his friend and teammate. While he continued to deny his attraction as being anything more than platonic, he thanked Larry for his care and concern on the subject.
Rendezvousing with Steele, Challis, Stone, and Farr in The Tunnel of Souls, they were confronted by The Night Nurse as they began making their escape. Momentarily petrified by her presence, her attack on Rowland angered him so much that he fought back against Death's enforcer. Telling the demon that they were not returning to Hell anytime soon, so long as they had people still on Earth who needed their help, Edwin proudly proclaimed that he was no longer scared of her.
Thanks to the sacrifice of The Spirit of Malcolm DuPont, in an act of love and devotion to his former lover and teammate "Bendy," Payne led them all to the safety of Charon's ferry. Sheltered from any further threat, it was not long before they were all back in their own bodies on the mortal plane.
Reunited with Crystal and Dorothy, who had taken a shine to one another during the mission, he agreed to allow Spinner to join them as another member of the agency. Saying goodbye to the team, they departed the mansion, listening to Rowland's jokes that his pessimistic, severe, and moody character was like that of "Goggles" (Larry). While he batted away the snark, he could not argue that despite their initial disastrous interactions, he and Trainer had grown friendlier with their time together.
Though The Night Nurse covered him and Charles with demonic zombifying vomit, the lack of an organic body/body parts prevented them from transforming into zombies; a fate that befell the other members of the Afterlife escape party.